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目的研究曲克芦丁对中波紫外线(UVB)诱导的HaCaT细胞光损伤的保护作用及机制。方法将培养的HaCaT细胞分为空白对照组、UVB组、曲克芦丁组、曲克芦丁+UVB组。其中UVB组、曲克芦丁+UVB组予50m J/cm~2的UVB照射,曲克芦丁组、曲克芦丁+UVB组分别加入不同浓度的曲克芦丁干预。用CCK-8法检测细胞增殖能力。Hoechst染色法检测细胞凋亡。Western blot法检测丝裂原活化蛋白激酶(MAPK)信号通路相关蛋白p-P38,p-JNK,p-ERK,以及激活蛋白-1(AP-1)的组件c-Fos,c-Jun的表达情况。结果 CCK-8法提示,5~10μmol/L的曲克芦丁对UVB诱导的HaCaT细胞光损伤有较好的保护作用(P<0.05)。Hoechst染色显示,UVB组凋亡细胞较空白对照组增多,曲克芦丁+UVB组凋亡细胞较UVB组减少。Western blot法提示UVB照射后HaCaT细胞p-P38,p-ERK,p-JNK,p-c-Jun及c-Fos水平升高,曲克芦丁+UVB组有不同程度下降。结论曲克芦丁对UVB诱导的HaCaT细胞光损伤有一定的保护作用。
Objective To study the protective effect and mechanism of troxerutin on photodamage induced by UVB in HaCaT cells. Methods The cultured HaCaT cells were divided into blank control group, UVB group, troxerutin group and troxerutin + UVB group. The UVB group and the troxerutin + UVB group were irradiated with UVB at a dose of 50m J / cm ~ 2. Troxerutin, troxerutin and UVB groups were treated with different concentrations of troxerutin. CCK-8 method was used to detect cell proliferation. Hoechst staining was used to detect apoptosis. Western blot was used to detect the expression of c-Fos and c-Jun in the mitogen-activated protein kinase (MAPK) signaling pathway related proteins p-P38, p-JNK, p-ERK and activator protein-1 Happening. Results CCK-8 method suggested that 5 ~ 10μmol / L troxerutin had better protective effect on UVB-induced HaCaT cells photodamage (P <0.05). Hoechst staining showed that apoptotic cells in UVB group increased compared with blank control group, while apoptotic cells in troxerutin + UVB group decreased compared with UVB group. The results of Western blot showed that the levels of p-P38, p-ERK, p-JNK, p-c-Jun and c-Fos in HaCaT cells increased after UVB irradiation, and decreased in the Troxerutin + UVB group. Conclusion Troxerutin has some protective effects on UVB-induced HaCaT cells photodamage.