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This study for the first time proposed an efficient microbial electrolyte/UV system for Methyl Orange decomposition.With an extal applied voltage of 0.2 V and cathode aeration of 20 mL/min,H2O2 could be in-situ generated from two-electron reduction of oxygen in cathode,reaching to 8.1 mg/L in 2 hr and continued to increase.The pollutant removal efficiency of approximate 94.7% was achieved at initial neutral pH,with the activation of·OH in the presence of UV illumination.Although the nature of its guiding prinicples remain on the vista of practical exploration,this proof-of-concept study provides an altemative operation patt of solar-microbial hybrid technology for future wastewater treatment from a basic but multidisciplinary view.