反义技术即用人工合成或生物合成的特定互补的ＤＮＡ或ＲＮＡ片段阻断从基因到蛋白的信息流，以达到抑制或阻断基因的表达。利用这一技术可以从分子水平进一步阐明许多神经生物学现象的机理。近年来反义技术及其在神经生物学中的应用发展较快。如对用常规手段很难研究的脑内神经肽Ｙ，利用反义技术可以更深入地了解它的功能特点，其他如在神经系统中ＮＭＤＡ受体、阿片受体以及ｃ－ｆｏｓ功能的研究中，反义技术都有广泛的应用。 Antisense technology is the use of artificial synthetic or biosynthesis of specific complementary DNA or RNA fragments blocking the flow of information from the gene to the protein in order to achieve the suppression or block gene expression. The use of this technique can further elucidate the mechanisms of many neurobiology phenomena at the molecular level. In recent years, antisense technology and its application in neurobiology developed rapidly. As in the brain neuropeptide Y difficult to study by conventional means, its functional characteristics can be further understood by using antisense technology. Others, such as studies on NMDA receptors, opioid receptors and c-fos function in the nervous system , Antisense technology has a wide range of applications.