人体DNA限制性片段长度多态性的发现和应用,使遗传多态性的分析从原来的血型、血清蛋白型和酶型进入基因的分子水平。从人类有核细胞中将DNA分离出来,利用核酸限制性内切酶在DNA特定顺序的识别位点上进行酶切而得到不同长度的片段,通过电泳分离、Southern印迹转移,然后用一种特殊的放射性 The discovery and application of human DNA restriction fragment length polymorphism allows the analysis of genetic polymorphisms from the original blood type, serum protein type and enzyme type into the molecular level of the gene. DNA is isolated from human nucleated cells, digested with restriction endonucleases at DNA-specific recognition sites to give fragments of different lengths, isolated by electrophoresis, transferred to Southern blots, and then probed with a special Of radioactivity