Trapidil是一种三唑嘧啶的衍生物,直接作用于前列环素(PGI_2)和血栓素(TXA_2)合成酶从而选择性抑制TXA_2的同时促进PGI_2的生成。为研究Trapidil在胚胎组织中对 PGI_2/TXA_2比值的影响。选用人脐静脉离体灌流的方法进行实验。分娩后立即收集20个健康新生儿脐静脉浸泡在冰冷的克氏—汉氏液中,冲洗之后节取中间部分10cm长的段,从两端插入套管,放入灌流室里并与一个恒速蠕动泵相连。实验要求在取材后1h内开始。在测药物、之前,用37℃含有95％O_2和5％CO_2的克氏—汉氏 Trapidil is a triazolopyrimidine derivative that acts directly on PGI_2 and TXA_2 synthase to selectively inhibit TXA_2 and promote PGI_2 production. To investigate the effect of Trapidil on PGI2 / TXA2 ratio in embryonic tissues. Human umbilical vein perfusion method for experiments. Immediately after childbirth, twenty healthy newborn infants were drowned in cold K-Hance solution. After washing, the mid-section of 10 cm long was sectioned, cannulas were inserted from both ends into the perfusion chamber and incubated with a constant Peristaltic pump connected. Experimental requirements in the first 1h after drawing. Prior to testing the drug, K-Han’s containing 95% O 2 and 5% CO 2 at 37 ° C were used