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通过甲基脂肪酸气相色谱分析法 (GC- FAME)、Biolog细菌鉴定法 (借助于计算机的数值分类法 )和其它试验对黄瓜苗期猝倒病具有明显防治效果的 16株拮抗菌株进行分类鉴定 .结果表明 :黄瓜苗期猝倒病拮抗菌的优势种为 Pseudomonas aeruginosa和 Bacillus brevis,其次为 Stenotrophomonas mal-tophilia,B.polymyxa,Pseudomonas fluorescens和 Agrobacterium radiobacter及一些还有待进一步鉴定的种 .并对 GC- FAME和 Biolog的鉴定结果进行了比较 :在属的水平上两种方法鉴定一致的拮抗菌株为 14株 ,占 87.5 % ;在种的水平上鉴定一致的拮抗菌株为 7株 ,占 4 2 .75 % .GC- FAME和 Biolog对某些细菌的鉴定提供了快速而可靠的方法 ,但由于目前计算机数据库尚不完备 ,鉴定部分细菌如 B.subtilis需用其它补充方法
Sixteen antagonistic strains were identified by GC-FAME, Biolog bacterial identification (by computer numerical classification) and other experiments on cucumber seedling damping-off disease. The results showed that the dominant species of antagonistic bacteria of Cucumber seedling damping-off disease were Pseudomonas aeruginosa and Bacillus brevis, followed by Stenotrophomonas mal-tophilia, B.polymyxa, Pseudomonas fluorescens and Agrobacterium radiobacter and some species to be further identified, The results of FAME and Biolog were compared: 14 strains (87.5%) of the antagonistic strains were identified by the two methods at the genus level, 7 strains were identified as antagonistic strains at the species level, accounting for 42.275 % .GC-FAME and Biolog provide a quick and reliable method for the identification of certain bacteria. However, due to the current incomplete database of bacteria, identification of some bacteria such as B.subtilis requires additional methods