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目的探讨微小RNA-143(miRNA-143)在非小细胞肺癌(NSCLC)中的表达及其细胞生物学功能。方法采用qRT-PCR检测miRNA-143在50例NSCLC组织及相应癌旁组织中的表达水平;脂质体介导的转染方法将miRNA-143模拟及miRNA-143抑制剂转染A549细胞;细胞计数试剂盒和细胞划痕实验分别检测miRNA-143对细胞增殖及迁移的影响;荧光素酶报告基因及Western blot分析miRNA-143的作用靶点。结果 miRNA-143在66.0%(33/50)的NSCLC组织中表达;其相对表达量低于癌旁组织(0.84±0.12vs.2.77±0.34)(P<0.05)。miRNA-143可以促进A549细胞的增殖及迁移,可以靶向胰岛素样生长因子1受体(IGF1R)的3’UTR端并抑制IGF1R蛋白的表达。结论 miRNA-143在NSCLC中低表达,能抑制NSCLC增殖及迁移;其机制可能与靶向调节IGF1R基因并抑制其蛋白的表达有关。
Objective To investigate the expression of microRNA-143 (miRNA-143) in non-small cell lung cancer (NSCLC) and its biological functions. Methods The expression of miRNA-143 was detected by qRT-PCR in 50 NSCLC tissues and its corresponding paracancerous tissues. The miRNA-143 mimics and miRNA-143 inhibitor were transfected into A549 cells by liposome-mediated transfection. Counting kit and cell scratch assay were used to detect the effect of miRNA-143 on cell proliferation and migration respectively. Luciferase reporter gene and Western blot were used to analyze the target of miRNA-143. Results The miRNA-143 was expressed in 66.0% (33/50) of NSCLC tissues. The relative expression level of miRNA-143 was lower than that in adjacent non-cancerous tissues (0.84 ± 0.12 vs. 2.77 ± 0.34) (P <0.05). miRNA-143 can promote the proliferation and migration of A549 cells, which can target the 3’UTR end of insulin-like growth factor 1 receptor (IGF1R) and inhibit the expression of IGF1R protein. Conclusion The low expression of miRNA-143 in NSCLC can inhibit the proliferation and migration of NSCLC. The mechanism may be related to the regulation of IGF1R gene expression and the inhibition of its expression.