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采用聚乙二醇（PEG）沉淀法纯化克隆刚地弓形虫（Toxplasmagondii）p30基因的转移载体质粒pSXIVVI+X3－p30DNA；将pSXIVVI＋X3－p30DNA与粉纹夜蛾核型多角体缺陷型病毒TnNPV－SVI－GDNA共转染草地夜蛾（Sfg）细胞，构建出既能形成多角体又能表达外源基因的重组病毒TnNPV－p30。经空斑纯化挑选出6株单克隆重组病毒，感染细胞裂解液在相对分子质量约为34000～35000位置出现一条表达带；其含量占细胞总蛋白的5.13％～5．62％，株间差异不明显；用抗弓形虫多克隆抗血清进行Western－Blot呈一特异反应条带。感染后72h蛋白表达量最高，可达7．18％；96小时次之，为5．91％。
The plasmid pSXIVVI + X3-p30 was cloned by polyethylene glycol (PEG) precipitation method. The pSXIVVI + X3-p30DNA was cloned into Toxoplasma gondii nuclear polyhedrosis virus TnNPV-SVI -GDNA were co-transfected with Sfg cells to construct a recombinant virus TnNPV-p30 that can form both polyhedrosis and foreign genes. Six strains of monoclonal recombinant virus were selected by plaque purification, and one expression band was found in the cell lysate from about 34000-35000 with the content of 5.13-5.62% of the total cellular protein. The difference between strains Obvious; Western-Blot with anti-Toxoplasma polyclonal antiserum showed a specific reaction band. 72h after infection the highest protein expression, up to 7.18%; 96 hours followed by 5.91%.