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目的探讨卵巢癌组织中M2型巨噬细胞的分布及其对卵巢癌侵袭转移相关基质金属蛋白酶(MMPs)表达的影响。方法免疫组织化学法检测上皮性卵巢癌(34例)、交界性卵巢肿瘤(16例)和卵巢良性肿瘤(18例)组织切片中CD68、CD206和MMP-9的表达,比较卵巢癌样本中阳性表达细胞数与患者年龄、病理类型、FIGO分期、组织学分化和淋巴结转移的关系。并利用流式细胞术比较其中12例上皮性卵巢癌和11例卵巢良性肿瘤新鲜组织中CD68+~CD206+~细胞比例。实时荧光定量聚合酶链式反应(Real-time PCR)和蛋白免疫印记(Western blot)方法分别评价卵巢癌和卵巢良性肿瘤组织内MMP-2、MMP-9和MMP-10水平。结果卵巢癌组织中CD68+~、CD206+~和MMP-9+~细胞数量明显高于交界性卵巢肿瘤和卵巢良性肿瘤组织(P<0.05),卵巢癌组织中CD68+~、CD206+~和MMP-9+~细胞数与患者FIGO分期和淋巴结转移均有统计学关系(P<0.05)。卵巢癌组织中CD68+~和CD206+~细胞数量呈正相关关系(r=0.508,P<0.05),且MMP-9+~与CD68+~和CD206+~细胞数量也分别呈正相关(r=0.611,P<0.001;r=0.744,P<0.001)。卵巢癌新鲜组织中CD68+~CD206+~细胞比例明显高于良性卵巢肿瘤(P<0.05),且卵巢癌组织MMP-2、MMP-9和MMP-10 m RNA和蛋白表达水平较卵巢良性肿瘤具有统计学意义(P<0.05)。结论卵巢癌组织中具有较高水平的M2型巨噬细胞,与卵巢癌侵袭转移和临床进展有重要的联系。
Objective To investigate the distribution of M2 macrophages in ovarian cancer and its effect on the expression of matrix metalloproteinases (MMPs) in ovarian cancer. Methods The expressions of CD68, CD206 and MMP-9 in epithelial ovarian cancer (34 cases), borderline ovarian tumor (16 cases) and benign ovarian tumor (18 cases) were detected by immunohistochemistry. The relationship between the number of expressed cells and patient’s age, pathological type, FIGO stage, histological differentiation and lymph node metastasis. The proportion of CD68 + CD206 + cells in fresh tissues of 12 cases of epithelial ovarian cancer and 11 cases of ovarian benign tumor was compared by flow cytometry. Real-time PCR and Western blot were used to evaluate the levels of MMP-2, MMP-9 and MMP-10 in ovarian cancer and benign ovarian tumor respectively. Results The numbers of CD68 +, CD206 + and MMP-9 + cells in ovarian cancer tissues were significantly higher than those in borderline ovarian tumors and benign ovarian tumor tissues (P <0.05). The expressions of CD68 +, CD206 + and MMP- There was a statistically significant relationship between ~ ~ cell number and FIGO stage and lymph node metastasis (P <0.05). There was a positive correlation between the number of CD68 + ~ and CD206 ~ ~ cells in ovarian cancer tissues (r = 0.508, P <0.05), and the numbers of MMP-9 + ; r = 0.744, P <0.001). The proportion of CD68 + ~ CD206 ~ ~ cells in fresh ovarian tissues was significantly higher than that in benign ovarian tumors (P <0.05), and the expression of MMP-2, MMP-9 and MMP-10 mRNA and protein in ovarian cancer was statistically higher than that in benign ovarian tumors Significance (P <0.05). Conclusion Ovarian cancer tissue with high levels of M2 macrophages, and invasion and metastasis of ovarian cancer and clinical progress have an important connection.