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人类疟原虫红外期的生活周期较长,其体外培养的主要障碍是如何延长单层肝细胞的保存时间。本文报导经丙种射线照射过的人肝细胞在体外生长至少能维持5周,对疟原虫子孢子的侵入与不经照射的肝细胞同样敏感,并产生相当数量具感染性的红外期裂殖子。体外培养使用HepG_2-A16人肝细胞,置于含10%小牛血清与加入双抗的RPMI1640培养基中的盖玻片上,在37℃含5%CO_2的空气中培养,2～3天后,肝细胞连合成单层,用钴60照射,照射剂量分别为1,000、3,000、6,000、9,000和12,000rad。照射后更换培养基再培养24小时,每份经照射的和非照射
Plasmodium falciparum infrared life cycle is longer, its main obstacle in vitro culture is how to extend the preservation of monolayer of liver cells. This paper reports that human liver cells irradiated with gamma ray irradiation can be maintained in vitro for at least 5 weeks. Infection of P. falciparum sporozoites is equally sensitive to non-irradiated hepatocytes and results in a considerable number of infectious merozoites . HepG2-A16 human hepatocytes were cultured in vitro and placed in glass coverslips containing 10% fetal bovine serum and RPMI 1640 medium supplemented with double antibody and cultured in air containing 5% CO 2 at 37 ° C. After 2 to 3 days, the liver The cells were united into a monolayer and irradiated with cobalt 60 at doses of 1,000, 3,000, 6,000, 9,000 and 12,000 rad, respectively. After irradiation, the media was changed and incubated for an additional 24 hours, each irradiated and non-irradiated