目的:通过对胰腺肿瘤组织和对应的癌旁正常组织的Midkine(MK)mRNA定量检测、比较分析,为胰腺癌的早期诊断建立分子标志。方法:收集胰腺癌组织及癌旁组织人体标本,冷冻保存;提取组织总RNA。根据MK两端序列设计特异性引物,同时用β-肌动蛋白作为对照基因,逆转录PCR(RT-PCR)和荧光定量逆转录PCR(qRT-PCR)对MKmRNA分别进行定性和定量测定。结合统计学分析结果,判断分析MKmRNA含量和胰腺癌的相关性。结果:胰腺癌组织中MKmRNA表达明显高于癌旁组织(p<0.05)。结论:基于与胰腺癌的相关性,MK有可能作为胰腺癌早期诊断的分子标记。 OBJECTIVE: To establish a molecular marker for the early diagnosis of pancreatic cancer through quantitative detection and comparative analysis of Midkine (MK) mRNA in pancreatic tumor tissues and corresponding normal tissues. Methods: Human pancreatic cancer tissues and adjacent tissues were collected and cryopreserved. Tissue RNA was extracted. Specific primers were designed according to the two-terminal sequence of MK, and β-actin was used as a control gene. The mRNA of MK was quantitatively determined by reverse transcription-PCR (RT-PCR) and quantitative reverse transcription-PCR (qRT-PCR). Combined with statistical analysis results, to determine the correlation between MKmRNA content and pancreatic cancer. Results: The expression of MK mRNA in pancreatic cancer tissues was significantly higher than that in adjacent tissues (p <0.05). Conclusion: Based on the association with pancreatic cancer, MK may be used as a molecular marker for the early diagnosis of pancreatic cancer.