针叶树组织培养,近年来虽然受到诸多学者的重视,获得可喜的进展,但与阔叶树相比:生长速度比较慢;丛生芽及试管苗的产量低;材料污染率比较高,培养基易于褐变;对培养基特别是植物激素在器官分化中的作用机理了解不够。因此,关于针叶树离体培养形态分化的机理还有待进一步研究。雪松[Cedrus deodara(Roxb.)G.Don]为世界著名的观赏和园林绿化树种之一。但北方的雪松产种量很低,用常规的营养繁殖法繁殖也比较困难。如果能通过组织培养法繁殖雪松,不仅可以保持母本的优良特性,而且可以在较短时间 Conifers tissue culture in recent years, although many scholars pay attention to obtain gratifying progress, but compared with the broad-leaved tree: the growth rate is relatively slow; clusters of buds and test tube seedlings yield is low; the rate of material contamination is relatively high, the medium is easy browning; Not enough understanding of the mechanism of action of the medium, especially plant hormones in organ differentiation. Therefore, the coniferous tree morphological differentiation in vitro mechanism remains to be further studied. Cedrus deodara (Roxb.) G.Don] is one of the world’s most famous ornamental and landscaping species. However, the yield of cedar in the north is very low and breeding by conventional vegetative propagation is more difficult. Cedar propagation through tissue culture, not only to maintain the excellent characteristics of the female parent, but also in a relatively short period of time