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目的 观察 3′ 酮基 去甲基苏氨酸1 缬氨酸2 环孢素 (SDZPSC 833,简称环孢素 )逆转宫颈癌细胞对丝裂霉素 (MMC)耐药性的效果。方法 以人宫颈癌Hela细胞及其耐药亚系 (Hela/MMC)细胞为材料 ,建立人类宫颈癌裸鼠异种移植瘤耐药模型 ,观察 1或 3mg/L环孢素于体内外对Hela/MMC细胞生长抑制的情况及细胞形态学变化 ;并观察体内应用环孢素后荷瘤裸鼠肿瘤体积及瘤组织病理学改变。结果 体外实验表明 ,无毒性剂量的环孢素可部分逆转Hela/MMC细胞对MMC的 5倍耐药性 ;体内实验表明 ,环孢素和MMC联合应用 ,对Hela/MMC细胞裸鼠移植瘤的抑制率为 83 18% ,明显高于单纯应用MMC的抑制率 35 79%。两者比较 ,差异有极显著性 (P <0 0 1)。结论 无毒性剂量的环孢素于体内外可逆转Hela/MMC细胞对MMC的耐药性 ,其作为耐药修饰剂可用于宫颈癌的化学药物治疗。
Objective To observe the effect of 3’-keto-nor-methyl-threonine-1 valine-2-cyclosporine (SDZPSC 833) on reversing the drug resistance of cervical cancer cells to mitomycin C (MMC). Methods Human cervical cancer Hela cells and its drug-resistant subline (Hela / MMC) cells were used as materials to establish a xenograft-resistant model of human cervical carcinoma in nude mice. The effects of 1 or 3 mg / L cyclosporine on Hela / MMC cell growth inhibition and morphological changes of cells; and observe the tumor volume and tumor histopathological changes of nude mice after cyclosporine injection in vivo. Results In vitro experiments showed that non-toxic doses of cyclosporine partially reversed the 5-fold resistance of Hela / MMC cells to MMC. In vivo experiments showed that cyclosporine combined with MMC could inhibit the growth of Hela / MMC cells in nude mice The inhibitory rate was 83 18%, which was significantly higher than that of MMC alone 35 79%. There was a significant difference between the two groups (P <0.01). Conclusion Cyclosporin at a non-toxic dose can reverse the drug resistance of Hela / MMC cells to MMC in vitro and in vivo. As a drug-resistant modifier, cyclosporine can be used in the chemotherapy of cervical cancer.