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分离和克隆人喉癌中新的相关基因将有助于揭示喉癌的易感性与癌变机制。运用mRNA差异显示法对 2例成人喉癌组织及配对癌旁正常组织的基因表达进行研究 ,分离到 3 5个差异显示片段 ;用反向Northern点杂交筛选到 6个差异片段 ,经克隆、测序和匹配分析 ,得 12条不同cDNA序列 ,其中 4条为新基因序列 ,另外 8条与已知基因高度同源。将 12条cDNA序列固定在膜上 ,用来自喉癌和配对正常组织的总cDNA探针与其杂交和差异RT PCR分析 ,鉴定了它们在喉癌组织和癌旁正常组织中差异表达。上述结果提示这些差异cDNA序列可能与喉癌发病密切相关。
Isolation and cloning of new related genes in human laryngeal cancer will help reveal the susceptibility and cancer mechanism of laryngeal cancer. The mRNA differential display method was used to study the gene expression of two adult laryngeal cancer tissues and paired adjacent normal tissues. 35 differential display fragments were isolated; six differential fragments were screened by reverse Northern blot and cloned and sequenced. And matching analysis yielded 12 different cDNA sequences, of which 4 were new gene sequences and the other 8 were highly homologous to known genes. Twelve cDNA sequences were immobilized on the membrane. The total cDNA probes from laryngeal carcinoma and paired normal tissues were used for hybridization and differential RT PCR analysis to identify their differential expression in laryngeal and adjacent normal tissues. The above results suggest that these differential cDNA sequences may be closely related to the incidence of laryngeal cancer.