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为探讨肾小球硬化机制，应用胶体金免疫电镜技术对重复阿霉累注射加一侧肾切除所诱发的进行性肾小球硬化模型中细胞外基质（ＥＣＭ）成份－Ⅲ型、Ⅳ型胶原、层粘连蛋白（ＬＮ）和纤维连结蛋白（ＦＮ）进行了定位、定量、定性的研究，并对肾小球基底膜（ＧＢＭ）和系膜区上述成份的正常分布及硬化过程中的变化作了比较。结果显示：正常鼠ＬＮ、ＦＮ在系膜区和ＧＢＭ中的含量有显著性差异（Ｐ＜０．０１），而Ⅳ型胶原则基本相同，这三种成份在ＧＢＭ分布有一定的规律。肾小球硬化过程中ＬＮ、ＦＮ、Ⅳ型胶原明显积聚，弥漫性肾小球硬化期与正常鼠相比，增加幅度为１．０～３．２倍（Ｐ＜０．０１）；肾小球硬化后期ＧＢＭ内不再显著增加，而系膜区内则持续增加．正常肾小球内不存在的Ⅲ型胶原在硬化早期无明显积聚，其后急聚增多至弥漫性肾小球硬化期。与对照组相比，ＧＢＭ与系膜区Ⅲ型胶原分别增加了１３．９及３２．３倍。我们用该技术证实了ＥＣＭ成份积聚是肾小球硬化的基本病理特征。Ⅲ型间质胶原从无至有，增加幅度最大，似提示与肾小球硬化有更直接、密切的关系。
In order to explore the mechanism of glomerulosclerosis, we used colloidal gold immunoelectron microscopy to study the expression of extracellular matrix (ECM) -Ⅲ and type Ⅳ collagen in progressive glomerulosclerosis model induced by repeated injection of apheresis plus one-sided nephrectomy , Laminin (LN) and fibronectin (FN) were studied by localization, quantification and qualitative analysis. The normal distribution of GBM and mesangial area and the changes during the hardening process A The results showed that the contents of LN and FN in normal rats were significantly different between mesangial area and GBM (P <0.01), while the type Ⅳ collagen was basically the same. The distribution of these three components in GBM had certain rules. During the course of glomerulosclerosis, LN, FN and type IV collagen accumulated significantly, and the range of increase was 1.0 to 3.2 times (P <0.01) compared with normal mice in diffuse glomerulosclerosis; Post-GBM ball no longer significantly increased within the latter, while the mesangial area continued to increase. Type III collagen, which is not found in normal glomerulus, showed no obvious accumulation in the early stage of sclerosis and then increased rapidly to diffuse glomerulosclerosis. Compared with the control group, GBM and mesangial collagen type Ⅲ increased by 13.9 and 32.3 times, respectively. We used this technique to demonstrate that accumulation of ECM is a fundamental pathological feature of glomerulosclerosis. Type III interstitial collagen from scratch, the largest increase, it may suggest a more direct and close relationship with glomerular sclerosis.