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目的:探讨miR-17-5p抑制物对于骨肉瘤细胞系SOSP_9607细胞增殖和凋亡的影响。方法:四甲基偶氮唑蓝(MTT)法测定细胞增殖,进一步计算抑制率,流式细胞仪测定细胞凋亡。将SOSP_9607细胞分为对照组和实验组,对照组分为阴性对照和正常细胞对照组。实验组采用miR-17-5p抑制物(hsa-miR-17-5pinhibitors)抑制SOSP_9607细胞内miR-17-5p的活性。结果:与对照组相比,实验组显著抑制SOSP_9607细胞的增殖,有明显的剂量依赖性(P<0.01)。随着浓度从50nmol/L逐渐增加至200nmol/L,抑制率逐渐增高(P<0.01)。实验组凋亡率(9.6±1.8)%与阴性对照组凋亡率(3.5±0.4)%相比明显增高(P<0.01)。结论:miR-17-5p抑制物通过抑制SOSP_9607细胞中miR-17-5p的活性对SOSP_9607细胞的增殖和凋亡发挥重要作用。
Objective: To investigate the effect of miR-17-5p inhibitor on the proliferation and apoptosis of osteosarcoma cell line SOSP_9607. Methods: MTT assay was used to determine the cell proliferation. The inhibitory rate was further calculated and the apoptosis was measured by flow cytometry. SOSP_9607 cells were divided into control group and experimental group, the control group was divided into negative control group and normal cell control group. In the experimental group, the miR-17-5p inhibitor (hsa-miR-17-5pinhibitors) was used to inhibit the activity of miR-17-5p in SOSP_9607 cells. Results: Compared with the control group, the experimental group significantly inhibited the proliferation of SOSP_9607 cells in a dose-dependent manner (P <0.01). With the concentration increasing from 50nmol / L to 200nmol / L, the inhibition rate gradually increased (P <0.01). The apoptosis rate of experimental group (9.6 ± 1.8)% was significantly higher than that of negative control group (3.5 ± 0.4)% (P <0.01). Conclusion: miR-17-5p inhibitor plays an important role in the proliferation and apoptosis of SOSP-9607 cells by inhibiting the activity of miR-17-5p in SOSP-9607 cells.