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目的探讨血液中毒鼠强的气相色谱-质谱检验效果。方法本次研究选择提取毒鼠强的常见有机溶剂共8种,并选择毒鼠强死亡现场采集血液样本,进了气相质谱联用仪(GC/MS)分析,比较不同溶剂的提取效率。此外,选择8份来源于健康人群(无毒鼠强接触史)的血液样品进行空白试验,测定回收率;采用离子监测模式检测毒鼠强中毒死亡者血液样品。结果 8份空白血液添加5 ug毒鼠强,显示平均回收率为95.2%,乙酸乙酯的回收率为92.7%,两者差异不显著,p>0.05,故将乙酸乙酯作为首选有机溶剂;本次研究选择毒鼠强标准品进行GC/MS分析,获得GC/MS图谱,并解析GC/MS峰的碎片m/z42(基峰)、m/z132、m/z240等;采用离子监测模式(SIM)检测毒鼠强中毒患者的血液样品,以高端碎片m/z212、m/z240作为参考依据得到GC/MS-SIM分析图谱。结论在司法检验中:乙酸乙酯可作为提取毒鼠强的首选有机溶剂;采用离子监测模式检测毒鼠强中毒死亡者血液样品的检测准确度较高,可作为首选分析方法。
Objective To investigate the effect of strong gas chromatography-mass spectrometry in blood poisoned rats. Methods In this study, 8 kinds of common organic solvents were selected to extract strong tetramine. Selected blood samples from tetramine poisoned rats were collected and analyzed by gas chromatography / mass spectrometry (GC / MS) to compare the extraction efficiency of different solvents. In addition, eight blood samples from healthy population (non-tetanus to strong history of exposure) were selected for blank test and the recovery rate was determined. The blood samples from the death of tetramine poisoning group were detected by ion monitoring mode. Results The results showed that the average recovery was 95.2% in 8 blood samples, and the recovery rate of ethyl acetate was 92.7%. There was no significant difference between the two groups (p> 0.05). Therefore, ethyl acetate was the most preferred organic solvent. In this study, we selected tetramine standard for GC / MS, GC / MS and GC / MS peak fragment m / z 42, m / z 132 and m / z 240. Using ion monitoring mode (SIM) was used to detect blood samples of tetramine poisoning patients. High-end fragment m / z212 and m / z240 were taken as reference to get GC / MS-SIM analysis. Conclusion In judicial examination, ethyl acetate can be used as the first choice organic solvent for extracting tetramine. Detecting blood samples from tetramine poisoning patients by ion monitoring is more accurate and can be used as the first choice method.