【摘 要】
:
The assembly of microtubules is essential for physiological functions of microtubules. Addition of microtubule-stabilizing reagents or microtubule "seeds" is us
【机 构】
:
Key Laboratory of Plant Physiology and Biochemistry of Ministry of Agriculture,Key Laboratory of Cel
【基金项目】
:
the National Key Basic Research Project;the National Science Foundation for Distinguished Young Scholars;国家自然科学基金
论文部分内容阅读
The assembly of microtubules is essential for physiological functions of microtubules. Addition of microtubule-stabilizing reagents or microtubule "seeds" is usually necessary for plant tubulin assembly in vitro, which hinders the investigation of plant microtubule dynamics. In the present note, highly purified plant tubulins have been obtained from lily pollen, a non-microtubule-stabilizing reagent or microtubule "seed" system for plant tubulin assembly has been established and the analysis of plant tubulin assembly performed. Experiment results showed that purified tubulin polymerized in vitro, and a typical microtubule structure was observed with electron microscopy. The kinetics curve of tubulin assembly exhibited typical "parabola". The presence of taxol significantly altered the character of plant tubulin assembly, including that abnormal microtubules were assembled and the critical concentration for plant tubulin assembly was decreased exceedingly from 3 mg/mL in the absence of taxol to 0.043 mg/mL in the presence of taxol.
其他文献
人类大量使用矿物资源,这在大力推进全球经济发展的同时也破坏了我们赖以生存的环境。如今,全球气候变暖给人类生存和生活带来了诸多弊端,因此,采取相关措施降低空气中的二氧化碳
近年来,随着人们生活水平的不断提高,人们的经济水平自然不在话下,此时的人们,对于身体健康的要求也自然而然的提升了,对于蔬菜水果的需求量也越来越大,而对于研究者而言,如何更好的
GATA-2,a transcription factor,is expressed in several types of blood cells and in the central nervous system (CNS),and regulates the differentiation of these ce
随着经济和科技的不断发展,石油等化工企业也得到了一定程度上的进步和完善。近年来,石油产业的发展在拉动经济增长的同时,也带来了一系列的环境问题。本文的主要内容就是对石油
Using modified cDNA RDA capitalizing on the high affinity of streptavidin for biotin and magnetic-absorption-based separation,we have obtained four bands of spe
分析了普通车床常见故障的原因及类型,并给出了故障排除的主要方法,这对普通车床的合理、安全的使用、维护和检修具有一定的借鉴和参考意义。
葡现在人们关于葡萄酒的酿制已经形成了成熟的工艺,并且根据不同的葡萄种类及不同的风味要求形成了不同的酿造方法。本文就葡萄酒酿造相关工艺进行了分析,主要包括酿造设备的相
A method using synchrotron radiation X-ray fluorescence analysis to in situ determine trace elements during protein electrophoretically separating process was e
当今世界最主要的环境问题就是气候问题。由于世界各国在发展经济的同时,没有从根本上解决环境污染问题,导致环境问题日益突出。当今世界面临着全球气候变暖、臭氧层破坏和损耗
随着我国矿山开采技术的快速发展,矿山在生产过程中会给矿区及周围自然环境、地质环境等各个方面带来一定影响和破坏。如何在矿山开采过程中对矿山地质环境进行保护与治理,已经