论文部分内容阅读
作者应用外源性DNA转染技术,以人鼻咽癌组DNA,在体外对NIH/3T3细胞进行转染实验。结果观察到在第1、2轮转染中,均获得明显转化灶。并以人Alu顺序为探针,经分子杂交证明1、2轮转化细胞DNA中含有人顺序。以转化细胞注入裸鼠皮下,能诱发形成纤维肉瘤。本实验表明人鼻咽癌DNA中存在具有转化活性的转化基因(癌基因),并已转移至细胞内,发生了明显的恶性转化。作者应用免疫组化技术,观察了Ha-ras基因产物p21蛋白质在人鼻咽癌中的表达。其结果表明,p21在人鼻咽癌中具有阳性表达,其阳性率为90.4％。
Using exogenous DNA transfection technology, the authors transfected NIH/3T3 cells with human nasopharyngeal carcinoma DNA in vitro. As a result, it was observed that in the first and second rounds of transfection, significant foci were obtained. Using the human Alu sequence as a probe, molecular hybridization revealed that the 1,2 round transformed cell DNA contained human sequences. Subcutaneous injection of transformed cells into nude mice can induce the formation of fibrosarcoma. This experiment shows that there is a transforming gene (oncogene) in the DNA of human nasopharyngeal carcinoma that has been transferred into the cell and that a significant malignant transformation has occurred. The authors applied immunohistochemical techniques to observe the expression of Ha-ras gene product p21 protein in human nasopharyngeal carcinoma. The results indicate that p21 has a positive expression in human nasopharyngeal carcinoma, with a positive rate of 90.4%.