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目的通过小干扰RNA技术(RNAi)敲低胃癌SGC7901细胞转移黏附蛋白(MTDH)即星形细胞上调基因1(AEG-1),并探讨其对SGC7901细胞增殖和迁移的影响。方法构建MDTH短发夹RNA(MTDH-sh RNA)质粒并转染SGC7901细胞,Western blot法检测SGC7901细胞MTDH蛋白水平。敲低SGC7901细胞MTDH水平后,MTT法检测细胞增殖情况,TranswellTM小室检测SGC7901细胞迁移能力。结果 MTDH-sh RNA转染SGC-7901胃癌细胞后,可明显敲低细胞中MTDH蛋白水平。敲低SGC7901细胞MTDH水平后,细胞的增殖和迁移能力均显著降低。结论敲低SGC7901细胞MTDH水平可抑制SGC-7901胃癌细胞的增殖和迁移。
OBJECTIVE: To knock down the expression of metastatic adhesion protein (MTDH) in gastric cancer SGC7901 cells by RNA interference and to study the effect of AEG-1 on the proliferation and migration of SGC7901 cells. Methods MDTH short hairpin RNA (MTDH-sh RNA) plasmid was constructed and transfected into SGC7901 cells. The MTDH protein level of SGC7901 cells was detected by Western blot. After MTDH was knocked down, the proliferation of SGC7901 cells was detected by MTT assay and the migration of SGC7901 cells was detected by TranswellTM chamber. Results MTDH-sh RNA transfected SGC-7901 gastric cancer cells, can significantly knock down MTDH protein levels in cells. After knocking down the level of MTDH in SGC7901 cells, the cell proliferation and migration ability were significantly reduced. Conclusion The knockdown of MTDH in SGC7901 cells can inhibit the proliferation and migration of SGC-7901 gastric cancer cells.