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为了明确北京顺义某锦鲤养殖场患病锦鲤的发病死亡原因,根据鲤鱼浮肿病毒基因的保守序列设计引物进行套式PCR扩增,产物经电泳后可见548 bp片段和180 bp片段的DNA条带,空白对照无扩增条带;套式PCR的产物进行测序,测序结果和Genbank公布的鲤鱼浮肿病毒H504株序列完全一致。结果显示这是由鲤鱼浮肿病毒引起的鲤鱼浮肿病,并通过进一步分析,提示鲤鱼浮肿病在北京地区已经有所扩散,应引起养殖者以及鱼病工作者的关注。
In order to clarify the cause of death of koi in a koi carp farm in Shunyi, Beijing, primers were designed according to the conserved sequence of carp edema virus to amplify the DNA fragments of 548 bp and 180 bp The PCR products of the nested PCR products were sequenced. The sequencing results were in good agreement with the sequence of the carp edema virus H504 strain released by Genbank. The results showed that this was a carp edema caused by carp edema virus, and further analysis showed that carp edema has spread in Beijing area, which should cause the concern of farmers and fish disease workers.