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目的:基于代谢组学方法研究异常胆液质证大鼠尿液中的差异性代谢组分,并通过差异性代谢组分结果探讨异常胆液质证的分子基础。方法:选用Wistar大鼠,通过干热环境、干热性饲料、慢性电刺激等多复合因素建立维吾尔医学异常胆液质载体动物模型,通过代谢组学方法检测尿液中的差异性代谢物。结果:异常胆液质证大鼠尿液具有显著性高峰度代谢物为异亮氨酸、亮氨酸、糖蛋白,具有显著性低峰度代谢物为谷氨酰胺、肌酸、柠檬酸、苯丙氨酸。结论:多因素复合刺激可促进异常胆液质的产生,异常胆液质证大鼠存在三大营养物质及能量代谢障碍,可能是异常胆液质证的分子基础。
OBJECTIVE: To study the differential metabolites of urine in abnormal bile-fluid-mass rats based on metabonomics and to explore the molecular basis of abnormal bile-fluid syndrome by differentially expressed metabolites. Methods: Wistar rats were used to establish the animal model of Uighur Abnormal bile-fluidic substance through multiple factors such as dry-heat environment, dry-heat feed and chronic electrical stimulation. Metabolomics was used to detect the differential metabolites in urine. Results: The urine samples of patients with abnormal bile-mass syndrome showed significant peak metabolites of isoleucine, leucine and glycoprotein with significant low-peak metabolites of glutamine, creatine, citric acid, benzene Alanine. Conclusion: Multiple factor stimulation can promote abnormal bilirubin production. There are three major nutritional substances and energy metabolism disorders in abnormal bile marrow syndrome rats, which may be the molecular basis of abnormal bile-fluid syndrome.