论文部分内容阅读
目的 探讨慢性肝病胆小管增生与肝纤维化的关系．方法 用ＬＤＰ 法观察７７ 例同时进行肝活组织学检查及血清纤维化标志检测的慢性肝病患者肝穿组织及１０ 例肝癌和２ 例正常人手术标本的ＣＫ－ １９ 单克隆抗体表达情况，并与肝病分级分期及血清纤维化标志等进行比较．结果 随ＣＫ－ １９ 表达强度从＋ 到＋＋＋＋ ，４ 项纤维化标志均逐步升高，尤其透明质酸与ＣＫ － １９ 表达强度的同步性最好，各组间比较均有显著性差异；ＣＫ － １９ 表达强度与肝病分级分期等显著相关（χ２ 分别为６５－６ ，６９－７ ，Ｐ 均＜ ０－０１） ；ＣＫ － １９ 染色还能显示胆小管破坏及淋巴细胞浸润．结论 ＣＫ－ １９ 染色对判断肝纤维化程度及肝炎相关的自身免疫现象有一定作用．
Objective To investigate the relationship between cholestatic tube proliferation and hepatic fibrosis in patients with chronic liver disease. Methods The LDP method was used to detect the expression of CK19 monoclonal antibody in liver biopsy tissues of 77 patients with chronic liver disease and 10 patients with hepatocellular carcinoma and 2 normal controls examined simultaneously by liver biopsy and serum fibrosis markers. Liver staging and serum fibrosis markers were compared. Results The four fibrosis markers gradually increased with the expression intensity of CK19 from + to ++++, especially the synchronicity of hyaluronic acid and CK - 19 expression intensity was the best among all groups. CK - The expression of 19 was significantly correlated with the grading and staging of liver diseases (χ2 = 65-6, 69-7, P <0-01, respectively). CK - 19 staining also showed the destruction of the bile duct and the infiltration of lymphocytes. Conclusion CK-19 staining may play a role in judging the degree of liver fibrosis and the autoimmune diseases related to hepatitis.