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目的 :复制大鼠高碳酸血症模型并探讨模型动物的某些病理生理改变。方法 :给大鼠呼吸高浓度二氧化碳气体 (8%CO2 ,2 1%O2 ,71%N2 ) ,每天 7h ,连续 2 8d。对比观察对照组 (A组 )和高碳酸血症组 (B组 )的各项指标变化。结果 :B组PaCO2 [(5 5 .90± 3.34 )mmHg],血浆、肺组织和右心室过氧化脂质 (LPO)显著高于A组 (P<0 .0 5 ) ;血红细胞、肺及右心室组织超氧化物歧化酶 (SOD)以及动脉血pH显著低于A组 (P <0 .0 5 )。平均右心房压( Rap)、平均右心室压 ( Rvp)和平均肺动脉压 ( Pap)两组间无显著性差异 ,但B组大鼠右心室重量明显高于A组 (P <0 .0 5 )。光镜下可见B组大鼠肺组织、右心室出现一系列损伤性改变。结论 :8%CO2 可以复制大鼠高碳酸血症模型。高浓度二氧化碳对机体具有一系列肯定的毒性致伤作用。临床上应用允许性高碳酸血症通气模式时应持高度慎重态度。
OBJECTIVE: To duplicate rat hypercapnia model and investigate some pathophysiological changes in model animals. Methods: Rats were exposed to high concentrations of carbon dioxide gas (8% CO2, 21% O2, 71% N2) for 7 h everyday for 28 days. The changes of each index of the control group (group A) and hypercapnia group (group B) were observed. Results: PaCO2 [(55.90 ± 3.34) mmHg], LPO in plasma, lung tissue and right ventricle were significantly higher in group B than those in group A (P <0.05) Right ventricular tissue superoxide dismutase (SOD) and arterial pH were significantly lower than those in group A (P <0.05). Mean right atrial pressure (Rap), mean right ventricular pressure (Rvp) and mean pulmonary artery pressure (Pap) were not significantly different between the two groups, but right ventricular weight was significantly higher in group B than in group A (P <0.05 ). Under light microscope, there was a series of damaging changes in lung tissue and right ventricle in group B rats. Conclusion: 8% CO2 can replicate rat hypercapnia model. High concentrations of carbon dioxide on the body has a series of positive toxicological injury. Clinical application of hypercapnia ventilation mode should be highly cautious attitude.