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目的 :建立糖尿病大鼠动物模型 ,通过大鼠糖尿病模型的保护实验 ,明确地肤根是否有降血糖作用和预防糖尿病肾病的作用。方法 :随机将糖尿病大鼠分成地肤根治疗组、消渴丸治疗组、生理盐水对照组各 2 0只 ,同期另设地肤根预防组 2 0只 ,共 80只。观察各组大鼠给药前后的血糖、尿糖、尿蛋白变化 ,及给药后各组血胰岛素、血脂、肾系数、肾脏病理的变化 ,并进行统计学分析。结果 :消渴丸组、地肤根组、预防组给药后 2周和 8周血糖均较给药前降低 (均 P <0 .0 0 1) ,实验结束后未出现尿蛋白 ;生理盐水组给药后 2周和 8周血糖与给药前比较差异均无统计学意义 (P >0 .0 5 ) ;给药后尿蛋白较给药前增加 (P <0 .0 1) ;给药后消渴丸组血糖下降优于地肤根组 (P <0 .0 5 ) ,而地肤根组血胰岛素水平高于消渴丸组 (P <0 .0 1) ;生理盐水组系膜区明显增宽 ,肾系数高于消渴丸、地肤根、预防组 (P <0 .0 1) ;消渴丸、地肤根、预防组均未出现肾脏病理损害 ,3组肾系数比较差异无统计学意义 (P >0 .0 5 ) ;给药后地肤根组甘油三酯较消渴丸组、生理盐水组低 (P <0 .0 5 )。结论 :地肤根有降血糖、预防糖尿病肾病的作用 ,并可能有降甘油三酯作用 ;地肤根降血糖机制可能是刺激胰岛 β细胞释放胰岛素
OBJECTIVE: To establish an animal model of diabetic rats and to determine whether there is a hypoglycemic effect on the root of the skin and the effect of preventing diabetic nephropathy by the protection experiment of the diabetic rat model. Methods: Diabetic rats were randomly divided into two groups: Kudzu root treatment group, Xiaoke Pill treatment group and saline control group, 20 rats in each group. The changes of blood glucose, urine sugar and urine protein before and after administration were observed and the changes of blood insulin, blood lipid, renal coefficient and renal pathology in each group were observed and statistically analyzed. Results: The blood glucose of Xiaoke Pill group, BDD group and preventive group were lower than those before administration (both P <0.01) at 2 weeks and 8 weeks after the administration. Urine protein did not appear after the experiment. There was no significant difference in blood glucose between the two weeks and 8 weeks after administration (P> 0.05), and the proteinuria in the two groups before administration increased (P <0.01) The decrease of blood glucose in Xiaokemao group was better than that in Hacutu root (P <0.05), while the level of insulin in Daodera group was higher than that in Xiaoque pills (P <0.01) The membrane area was obviously broadened, and the renal coefficient was higher than Xiaoke Pill, Kadsura root, prevention group (P <0.01); Kidney pathology was not found in Xiaoke Pill, The difference was not statistically significant (P> 0.05). After administration, the triglyceride of Rhizoma Dioscoreae group was lower than that of Xiaoke Pill group and saline group (P <0.05). Conclusion: Rhizoma Drynaria has hypoglycemic effect and prevents diabetic nephropathy, and may have triglyceride-lowering effect. The hypoglycemic mechanism of Rhizoma dexiruna may be to stimulate islet β-cell release of insulin