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目的:研究千里光Senecio scandens中的生物碱类化学成分。方法:采用硅胶和凝胶柱色谱进行分离,根据化合物理化性质和光谱数据鉴定其结构。采用MTT法对所得到的化合物进行原代小鼠肝细胞毒性检测。结果:分离得到6个化合物,分别为:阿多尼弗林碱(1),7-angeloylturneforcidine(2),大麦芽碱(3),1,3,6,6-四甲基-5,6,7,8-四氢异喹啉-8-酮(4),4-(吡咯烷-2-酮基)-苯基乙酸(5),4-吡咯烷基-苯基乙酸(6)。结论:化合物6为新天然产物,化合物3,4为首次从该属植物中分离得到,化合物2,5为首次从该植物中分离得到。毒性筛选结果表明,化合物1对原代小鼠肝细胞有细胞毒作用,其他化合物未表现出细胞毒作用。
Objective: To study the chemical constituents of alkaloids in Senecio scandens. Methods: The compounds were separated by silica gel and gel column chromatography. Their structures were identified by physicochemical properties and spectral data. The obtained compounds were subjected to primary mouse hepatotoxicity assay by MTT method. Results: Six compounds were isolated and identified as adoniflin base (1), 7-angeloylturneforcidine (2), barley malt (3), 1,3,6,6-tetramethyl-5,6 , 7,8-tetrahydroisoquinolin-8-one (4), 4- (pyrrolidin-2-onyl) -phenylacetic acid (5) and 4-pyrrolidinyl-phenylacetic acid (6). Conclusion: Compound 6 is a new natural product. Compounds 3 and 4 were isolated from this genus for the first time. Compounds 2 and 5 were isolated from this plant for the first time. Toxicity screening results showed that compound 1 had cytotoxic effects on primary mouse hepatocytes while other compounds did not show cytotoxicity.