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Objective: To investigate the molecular mechanism of Ruanjian Sanjie Capsules (软坚散结胶囊) through regulating NOTCH signaling pathway to promote human clear cell renal cell carcinoma 7860 cell apoptosis. Methods: A laser confocal microscope was used to observe the nuclear displacement of apoptosis-related proteins, and the expression of Notch1, NICD, Bcl-2, and Bax was detected by Western blotting (WB). Results: Laser confocal microscopy showed that the immunofluorescence intensity of Bcl-2 protein gradually decreased with the increase of Ruanjian Sanjie Capsules (软坚散结胶囊) drug-containing serum concentration, and the fluorescence intensity of Bax protein gradually increased with the increase of dose concentration, and it entered the nucleus to varying degrees; WB detection showed that Bcl-2, Notch1, and NICD protein expression levels were inversely correlated with the drug-containing serum dose concentration, that is, protein expression decreased with the increase of drug-containing serum concentration, while Bax protein expression increased gradually with the increase of serum concentration. Afte 7860 cells knock out Notch1, the expression of related proteins was consistent with the expression trend of the drug-containing serum group. Conclusion: Ruanjian Sanjie Capsule's (软坚散结胶囊) low, medium and high dose concentration of drug-containing serum can promote 7860 cell apoptosis, and it is concentration-dependent. The molecular mechanism of apoptosis is directly related with Ruanjian Sanjie Capsule (软坚散结胶囊) blocking Notch signaling pathway activation.