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Objective: To observe the synergistic effects of hyperthernia and cisplatin on human lung adenocarcinoma cell line H1299. Methods: Cells treated with or without hyperthermia at 42 ℃ for 2 h were detected cisplatin sensitivity with CCK-8 assay. After managed with hyperthermia at 42 ℃ for 2 h, ciplatin 10 μg/mL for 24 h or both of two sides, inhibition ratio was detected with CCK-8 assay and flow cytometry analyzed DNA contents in every phase of cell proliferation. Results: After hy- perthermia, IC50 values of cell to cisplatin decreased from 9.51 μg/mL to 6.07 μg/mL. CCK-8 assay showed hyperthermia and ciplatin had synergistic killing effects, and FCM also showed hyperthermia had significant effects on cell cycle with increase soef nGs0i/tGivi1t yp ohfa Hse1 2p9o9p uclealtl iolinne a tnod c irsepdluactitnio. n of S phase population. Conclusion: Hyperthermia can significantly increase the
After immunization with hyperthermia and cisplatin on human lung adenocarcinoma cell line H1299. Methods: Cells treated with or without hyperthermia at 42 ° C for 2 h were detected for cisplatin sensitivity with CCK-8 assay. for 2 h, ciplatin 10 μg / mL for 24 h or both of two sides, inhibition ratio was detected with CCK-8 assay and flow cytometry analyzed DNA contents in every phase of cell proliferation. Results: After hy- perthermia, IC50 values of cell to cisplatin decreased from 9.51 μg / mL to 6.07 μg / mL. CCK-8 assay showed hyperthermia and ciplatin had synergistic killing effects, and FCM also showed hyperthermia had significant effects on cell cycle with increase soef nGs0i / tGivi1t yp ohfa Hse1 2p9o9p uclealtl iolinne a tnod c irsepdluactitnio. n of S phase population. Conclusion: Hyperthermia can significantly increase the