目的 :检测人卵巢恶性肿瘤组织染色体微卫星 DNA序列的不稳定性并探讨其与临床病理特征的关系。方法 :应用 PCR-聚丙烯酰胺凝胶电泳 -硝酸银染色技术 ,检测 6 0例原发卵巢恶性肿瘤组织染色体微卫星 DNA序列的不稳定性。结果 :2 1例卵巢恶性肿瘤组织存在微卫星的不稳定 (35 .0 % ) ,微卫星的不稳定与肿瘤的病理分级、淋巴结转移及组织学类型有关 ,与患者的年龄、临床分期无关。结论 :微卫星 DNA序列的不稳定性可能在卵巢恶性肿瘤尤其是特殊类型肿瘤的发生过程中起较为重要的作用 ,并且与卵巢恶性肿瘤的不良预后有关 Objective: To detect the instability of chromosomal microsatellite DNA sequence in human ovarian cancer and to explore its relationship with clinicopathological features. Methods: PCR-polyacrylamide gel electrophoresis-silver nitrate staining was used to detect the chromosomal microsatellite DNA sequence instability in 60 primary ovarian malignant tumors. Results: Microsatellite instability (35.0%) was found in 21 cases of ovarian malignant tumor tissues. The microsatellite instability was related to tumor grade, lymph node metastasis and histological type, but not to patient’s age and clinical stage. Conclusion: The instability of microsatellite DNA sequence may play an important role in the development of ovarian malignancies, especially in specific types of tumors, and is associated with the poor prognosis of ovarian malignant tumors