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目的:了解疟原虫的多胺代谢与咯萘啶(PND)抗药性的关系。方法:感染伯氏疟原虫ANKA株(PS)和由该株培育的中抗PND品系(PRA)及高抗PND品系(PRB)的昆明株小鼠于腹腔接种(ip)后d7取血,经薄层层析后用荧光分光光度法测定正常RBC、PS、PRA和PRB感染RBC的丁二胺(PTC)、精脒(SPD)和精胺(SPM)量。另有感染PS和PRB的小鼠于ip后d6分别1次灌胃(ig)PND5mg/kg和10mg/kg,d7取血,按上述方法测定给药后感染RBC的多胺量,并与不给药组比较。结果:PS感染RBC的多胺量均明显高于未感染疟原虫的正常RBC,而感染PRA和PRB的RBC多胺量又显著高于PS感染RBC,且多胺量的增高与抗性程度有关。经PND治疗后PS感染RBC的SPD和SPM较未治疗组显著下降,而PRB感染RBC则未见明显变化。结论:伯氏疟原虫对PND的抗药性与其多胺代谢有关。
Objective: To understand the relationship between polyamine metabolism of Plasmodium and resistance to pyronaridine (PND). Methods: The Kunming strain of mice infected with Plasmodium berghei ANKA (PS), the medium resistant PND strain (PRA) and the high resistant PND strain (PRB) were inoculated intraperitoneally (ip) Thin layer chromatography was used to determine the amount of butanediamine (PTC), spermidine (SPD) and spermine (SPM) in RBCs infected with normal RBC, PS, PRA and PRB by fluorescence spectrophotometry. In addition, mice infected with PS and PRB were dosed intragastrically (ig) with PND 5 mg / kg and 10 mg / kg once a day d ip, respectively, and d7 blood was collected. The amount of polyamine in RBC after administration was determined as above, Administration group comparison. Results: The amount of polyamine in RBC infected with PS was significantly higher than that in normal RBC without P. falciparum infection, while the amount of polyamine in RBC infected with PRA and PRB was significantly higher than that in PS-infected RBC, and the increase of polyamine was related to the degree of resistance . Compared with untreated group, the SPD and SPM of PS-infected RBC decreased significantly after PND treatment, but there was no obvious change of PRB-infected RBC. Conclusion: The resistance of Plasmodium berghei to PND is related to polyamine metabolism.