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目的 探讨钴纳米颗粒对破骨样细胞的毒性作用及生物学活性,分析钴纳米颗粒与骨溶解的关系.方法 2014年11月至2015年7月,在体外培养单核巨噬细胞(RAW264.7),经脂多糖(lipopolysaccharides,LPS)诱导为破骨样细胞,而后分别加入不同浓度(10、20、50、100 μmol/L)钴纳米颗粒和氯化钴,并在显微镜下观察细胞形态.对LPS诱导24h后的破骨样细胞进行分组,即钴纳米颗粒组(10、20、50、100 μmol/L浓度)、氯化钴组(10、20、50、100 μmol/L)和空白对照组,于2、4、8、24、48 h分别用4种浓度的钴纳米颗粒与氯化钴分别干预破骨样细胞进行甲基噻唑基四唑(methyl thiazoliumtetrazolium,MTT)生物活性实验,同时使用Q-PCR法测定CAⅡ、CatK基因mRNA的相对表达量.结果 随浓度(10、20、50、100μmol/L)及作用时间(2、4、8、24、48 h)的增加,钴纳米颗粒与氯化钴对破骨样细胞的增殖抑制率明显增加,并呈时间、浓度依赖关系,其中钴纳米颗粒对破骨样细胞的增殖抑制率明显高于氯化钴.不同浓度(10、20、50、100μmol/L)钴纳米颗粒与氯化钴用的CAⅡ、Cat K基因mRNA相对表达量均较空白对照组减少,但钴纳米颗粒在10～50μmol/L浓度,对破骨样细胞CAⅡ、CatK的相对表达量增加,而氯化钴则减少.结论 不同浓度钴纳米和氯化钴对破骨细胞的增殖和分化均有抑制作用,且钴纳米颗粒较氯化钴作用更显著,当钴纳米颗粒浓度在10～50μmol/L时,对破骨细胞CAⅡ、Cat K的相对表达量有增加趋势,而同浓度下氯化钴则抑制.“,”Objective To explore the toxicity and biological activity of cobalt nanoparticles (CoNPs) on osteoclasts,and to analyze the relationship between cobalt nanoparticles and osteolysis.Methods From November 2014 to July 2015,RAW264.7 cell was induced to osteoclast-like cell by LPS.Different concentrations of cobalt nanoparticles and cobalt chloride were added,and the cell morphology was observed under a microscope.24 h after induction on RAW264.7,cells were grouped into cobalt nanoparticles group (10,20,50,100 μmol/L),cobalt chloride group (10,20,50,100 mol/L) and control group.MTT assessment and Q-PCR were performed at 2 h,4 h,8 h,24 h,48 h post-treatment.Results With the increase of concentration (10,20,50,100 μmol/L) and the action time (2 h,4 h,8 h,24 h,48 h),the inhibition rate of cobalt nanoparticles and cobalt chloride on osteoclast like cells was significantly increased,and the inhibition rate of cobalt nanoparticles was higher.With different concentrations (10,20,50,100 μmol/L) of CoNPs and cobalt chloride,the relative expression of CAⅡ,Cat K gene mRNA expression decreased compared with the control group,when the concentration of CoNPs was in the range of 10-50 μ mol/L,the relative expression of CAⅡ and Cat K was increased,which was reduced in cobalt chloride group.Conclusion Different concentrations of cobalt nanoparticles and cobalt chloride can inhibit the proliferation and differentiation of osteoclasts,and cobalt nanoparticles is more pronounced,when the concentration of cobalt nanoparticles was 10-50 μmol/L,the relative expression of osteoclasts CAⅡ,Cat K increaseed,which was suppressed at the same concentration of cobalt chloride.