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采用饮食限量法,将昆明小鼠分三组:自由进食、八份进食和半量进食的小鼠分别进行了免疫功能、肠道菌群与寿命的观察。结果显示:八份饱进食组小鼠寿命最长,2年全部存活(100%),自由进食组次之(33%),半量进食组只到18个月就全部死亡。在免疫的检测中发现ANAE+数及Mφ吞噬试验,八份饱进食组最高,自由进食组次之,半量进食组最低,相差显著或非常显著。在肠道需厌O2菌的检测中,结果显示:克雷伯氏菌、双岐杆菌、乳杆菌八份饱进食组明显的高于其自由进食组(p<005)和半量进食组(p<001),提示长寿小鼠免疫功能的增强及部分细菌的增加和适量的饮食限量有着密切的关系。
Kunming mice were divided into three groups according to the diet limited method: mice with free eating, eight eating and half eating had immunological function, intestinal flora and life expectancy. The results showed that the eight full-fed mice had the longest lifespan, survived for 2 years (100%), followed by the free-eating group (33%), and all the mice in the half-dose group died within 18 months. ANAE + number and Mφ phagocytosis test were found in the immune test, eight were the highest eating group, the second was the lowest, the difference was significant or very significant. In the test of intestinal bacteria, the results showed that: Klebsiella, Bifidobacterium, Lactobacillus eight full eating group was significantly higher than the free-eating group (p <0 05) and the half-food group (P <0 01), suggesting that immune function in mice with enhanced longevity and the increase of some bacteria and the amount of diet has a close relationship.