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Objective To determine the interaction between 2450-MHz microwaves (MW) radiation and mitomycin C (MMC). Methods The synergistic genotoxic effects of low-intensity 2450-MHz microwave and MMC on human lymphocytes were studied using single cell gel electrophoresis (SCGE) assay (comet assay) and cytokinesis-blocked micronucleus (CBMN) test in vitro. The whole blood cells from a male donor and a female donor were either only exposed to 2450-MHz microwaves (5.0 mW/cm2) for 2 h or only exposed to MMC (0.0125 μg/mL, 0.025μg/mL, 0.05 μg/mL and 0.1 μg/mL) for 24 h; and the samples were exposed to MMC for 24 h after exposure to MW for 2 h. Results In the comet assay, the comet lengths ( 29.1μm and 25.9μm) of MW were not significantly longer than those (26.3μm and 24.1μm) of controls (P>0.05). The comet lengths (57.4 μm, 68.9 μm, 91.4 μm, 150.6μm and 50.6μm, 71.7μm, 100.1 μm, 145.1 μm) of 4 MMC groups were significantly longer than those of controls (P<0.01). The comet lengths (59.1μm, 92.3μm, 12
Methods To determine the interaction between 2450-MHz microwaves (MW) radiation and mitomycin C (MMC). Methods The synergistic genotoxic effects of low-intensity 2450-MHz microwave and MMC on human lymphocytes were studied using single cell gel electrophoresis (SCGE) assay The whole blood cells from a male donor and a female donor were either exposed only to 2450-MHz microwaves (5.0 mW / cm2) for 2 h or only exposed to MMC (0.0125 μg / mL, 0.025 μg / mL, 0.05 μg / mL and 0.1 μg / mL) for 24 h; and the samples were exposed to MMC for 24 h after exposure to MW for 2 h. Results In the comet assay, The comet lengths of (29.1 μm and 25.9 μm) of MW were not significantly longer than those (26.3 μm and 24.1 μm) of controls (P> 0.05). The comet lengths (57.4 μm, 68.9 μm, 91.4 μm, 150.6 μm and 50.6 μm, 71.7 μm, 100.1 μm, 145.1 μm) of 4 MMC groups were significantly longer than those of controls (P <0.01). The comet lengths (59 .1 μm, 92.3 μm, 12