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目的 复制冲击波和液体火箭推进剂中毒致冲毒复合伤的动物模型。 方法 健康雄性 Wistar大鼠 392只 ,随机分为 7组 :1冲击伤组 ;2偏二甲基肼 (U DMH)组 ;3四氧化二氮 (N2 O4)组 ;4冲击伤加 U DMH组 ;5冲击伤加 N2 O4组 ;6冲击伤加 U DMH及 N2 O4组 ;7对照组。每组动物又分为伤前、伤后 3、6、12、2 4、48、72 h7个时相组 ,每个小组 8只。 方法 冲击伤组用 BST- I型生物激波管致伤 ,冲击波超压为 471.5 k Pa,正压持续时间 5 5～ 6 0 m s。 UDMH及 N2 O4组分别吸入U DMH和 N2 O415 min,保持染毒柜中 U DMH的浓度为 80 0× 10 - 6 ,N2 O4的浓度为 43× 10 - 6 ,复合伤组动物则在冲击伤后 10 m in在染毒柜中吸入 UDMH和 (或 ) N2 O415 min,观察各组动物的临床状况并分别于 3、6、12、2 4、48、72 h时相活杀测定其生化指标和气血分析。 结果 动物观察和实验测定结果表明 ,上述方法复制的冲毒复合伤的动物模型具有与临床所见病人相近、稳定性、重复性好、经济实用等特点。 结论 利用生物激波管冲击和染毒柜染毒相结合的形式可以模拟复制现场液体火箭推进剂爆炸致冲毒复合伤的动物模型。这一模型的建立 ,对开展这一领域的研究具有重要的价值
OBJECTIVE: To establish an animal model of shock-induced compound injury caused by shock wave and liquid rocket propellant poisoning. Methods 392 healthy male Wistar rats were randomly divided into 7 groups: 1 shock injury group, 2 DMD group, 3 N2 O4 group, 4 shock injury plus U DMH group ; 5 impact injury plus N2 O4 group; 6 impact injury plus U DMH and N2 O4 group; 7 control group. Each group of animals was divided into pre-injury, post-injury 3,6,12,2 4,48,72 h7 phase group, each group of 8. Methods The BST-I bio-shock tube was used to injure the injured group. The shock wave overpressure was 471.5 kPa and the positive pressure duration was 55 ~ 60 m s. UDMH and N2 O4 groups inhaled U DMH and N2 O415 min, respectively. The concentration of U DMH in the treated cabinet was 80 × 10 -6 and the concentration of N 2 O 4 was 43 × 10 -6. After 10 mins inhalation of UDMH and / or N2 O415 min in the exposure cabinet, the clinical status of each group was observed and their biochemical indexes were determined at 3, 6, 12, 2, 48, And blood analysis. Results Animal observations and experimental results show that the above animal model of red-wound compound wounds has the characteristics of being similar to those seen in clinical practice, good in stability and repeatability, and economical and practical. Conclusion The combination of biological shock tube and poison cabinet poisoning can simulate the animal model of duplicated liquid poison rocket explosion caused by explosion of liquid rocket propellant. The establishment of this model is of great value to the research in this field