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目的 探讨血清14-3-3η蛋白联合抗环瓜氨酸肽(CCP)抗体对类风湿关节炎(RA)的诊断价值.方法 纳入185例因关节症状就诊的患者,其中RA患者89例(RA组)和其他风湿病患者96例(其他风湿病组),选取同期健康体检者100例作为对照组.采用酶联免疫吸附试验(ELISA)检测3组患者血清14-3-3η蛋白和抗CCP抗体水平,比较两者在诊断RA中的敏感度和特异度.结果 RA组、其他风湿病组及对照组血清14-3-3η蛋白、抗CCP抗体、RF水平及阳性率比较,差异均有统计学意义(P<0.05).RA组血清14-3-3η蛋白水平及阳性率均高于其他风湿病组和对照组,其他风湿病组血清14-3-3η蛋白水平及阳性率均高于对照组(P<0.05).血清14-3-3η蛋白联合抗CCP抗体诊断RA的特异度明显提高,但敏感度降低.抗CCP抗体阴性和阳性的RA患者中血清14-3-3η蛋白的阳性率分别为70.6%(24/34)、81.8%(45/55).结论 血清14-3-3η蛋白联抗CCP抗体能提高RA的诊断率.“,”Objective To explore diagnostic value of serum 14-3-3η protein combined with anti-cyclic citrullinated peptide(CCP)antibody on rheumatoid arthritis(RA).Methods One hundred and eighty-five patients who suffered from arthritic problems were included and there were 89 patients with RA(RA group)and 96 patients with other rheumatic diseases(other rheumatic diseases group). One hundred healthy people were selected as the control group.Levels of serum 14-3-3η protein and anti-CCP antibody in the three groups were detected by enzyme-linked immunosorbent assay(ELISA)and compared.Sensitivity and specificity of serum 14-3-3η protein and anti-CCP antibody on diagnosing RA were compared.Results Levels and positive rates of serum 14-3-3η protein,anti-CCP antibody and RF were significantly different among RA group,other rheumatic diseases group and control group(P<0.05). Levels and positive rates of serum 14-3-3η protein in RA group were higher than those in other rheumatic diseases group and control group,and were higher in other rheumatic diseases group than those in control group(P<0.05).Specificity of serum 14-3-3η protein combined with anti-CCP antibody in diagnosing RA increased obviously,but its sensitivity decreased. Positive rates of serum 14-3-3η protein in RA patients with negative and positive anti-CCP antibody were 70. 6%(24/34)and 81. 8%(45/55) respectively.Conclusion Serum 14-3-3η protein combined with anti-CCP antibody can increase the diagnostic rate of RA.