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2016年9月贵州省贵阳市发生严重的辣椒病毒病,症状复杂,主要表现为植株矮化,叶片黄化、花叶、皱缩、畸形以及枯死斑,果实有坏死斑等,根据症状难以判断病毒种类。本文采用小RNA深度测序技术对田间自然发病的2株辣椒标样进行了毒源鉴定,发现样品1由蚕豆萎蔫病毒2号(Broad bean wilt virus2,BBWV2)、辣椒脉斑驳病毒(Chilli veinal mottle virus,ChiVMV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)和辣椒内源RNA病毒(Bell pepper endornavirus,BPEV)4种病原复合侵染;样品2中除鉴定到上述4种病毒外,还检测到马铃薯Y病毒(Potato virusY,PVY)。进一步通过反转录PCR(RT-PCR)对深度测序结果进行了验证,证明其准确可靠。其中4个辣椒标样中均有的辣椒内源RNA病毒(BPEV)为贵州省首次报道。多种病毒复合侵染是辣椒产量和品质的重要限制因素之一,是辣椒生产的主要威胁。
September 2016 Guiyang City, Guizhou Province, a serious pepper virus disease, the symptoms are complex, the main manifestations of plant dwarfing, leaf yellowing, mosaic, wrinkles, deformities and dead spots, dead spots and other fruits, according to the symptoms difficult to judge Virus types. In this study, two pepper samples were identified by natural microinverting DNA sequencing. The results showed that sample 1 was composed of Broad bean wilt virus 2 (BBWV2), Chilli veinal mottle virus , ChiVMV, Cucumber mosaic virus (CMV) and Bell pepper endornavirus (BPEV). In addition to the four viruses identified in sample 2, Potato virus Y (PVY). The results of deep sequencing were further verified by reverse transcription PCR (RT-PCR), which proved to be accurate and reliable. Among them, peppers endogenous RNA virus (BPEV) was reported in four pepper samples for the first time in Guizhou Province. Multiple virus infection is one of the most important limiting factors for pepper production and quality, and is the main threat to pepper production.