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对 5种鉴定小麦背景中 1BL/ 1RS易位染色体的生化和分子标记方法 ,包括酸性聚丙烯酰胺凝胶电泳 (A PAGE)、荧光原位杂交 (FISH)、RFLP、RAPD和特异性PCR标记 ,进行比较研究。结果表明 ,RAPD标记难以鉴定1BL/ 1RS易位染色体 ,其余均能对 1BL/ 1RS易位染色体进行有效鉴定。FISH和RFLP方法比较费时费力且花费昂贵 ,而特异性PCR标记又在一定程度上受模板DNA浓度的影响。据此认为 ,APAGE方法是一种简单、快速、经济有效的方法 ,最易于在小麦育种选择中应用
The five biochemical and molecular markers for the identification of 1BL / 1RS translocation chromosomes in the wheat background, including acidic polyacrylamide gel electrophoresis (A PAGE), fluorescence in situ hybridization (FISH), RFLP, RAPD and specific PCR markers, Make a comparative study. The results showed that it was difficult to identify 1BL / 1RS translocation chromosomes by RAPD markers, and the rest could be used to identify 1BL / 1RS translocation chromosomes effectively. The FISH and RFLP methods are time-consuming and expensive and the specific PCR markers are to some extent affected by the template DNA concentration. Therefore, APAGE method is a simple, rapid and cost-effective method that is most easily applied in wheat breeding selection