随着生物技术的发展,带有目的性状基因的总DNA及目的基因重组分子已被提取出来,由此派生的分子育种,也由试验探索向生产应用逐步发展。以离体组织细胞为受体的技术,目前在欧美国家进展较快,如Ti质粒载体法、电激法、显微注射法等,在双、单子叶植物中均获得转化植株,但尚未进入生产应用。而以整体细胞为受体的技术,已开始进入应用阶段。近年来,Hess,Ohta、Pena等分别报道了用微量注射法和以花粉为载体,获得了变异的新个体。我国周光宇、黄骏麒、陈善葆和张孔恬等,以花粉管通 With the development of biotechnology, the total DNA with the trait gene of interest and the recombinant gene of the target gene have been extracted. The molecular breeding derived therefrom has also been gradually developed from experimental exploration to production and application. In vitro tissue cells as the receptor technology, the current rapid progress in Europe and the United States, such as the Ti plasmid vector method, electroporation, microinjection, etc., obtained in both double and monocot plants transformed plants, but not yet entered Production applications. The whole cell receptor technology, has begun to enter the application stage. In recent years, Hess, Ohta and Pena et al. Have reported on the new individuals obtained by microinjection and pollen as vectors, respectively. China’s Zhou Guangyu, Huang Jun Yee, Chen Shanbao and Zhang Kong Tian, ​​etc., to pollen tube pass