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[Objective] To study various factors that affect the rapid propagation of wolfberry (Lycium barbarum L.) in vitro and to establish the rapid propagation system of wolfberry superior strains with the purpose of breeding a large number of high-quality seedlings. [Method] Using Ningqi No.1 twigs as explants, the sterilization methods, lateral bud induction, subculture, rooting culture mediums of wolfberry were optimized. Meanwhile, the transplanting medium of test-tube plantlet were optimized. [Result] Explants were first soaked for 5-8 min in 500 mg/L penicillin injecta then sterilized. The optimized culture mediums were MS+ 0.5 mg/L 6-BA+ 0.1 mg/L IAA for lateral bud induction, MS+ 0. 5 mg/L 6-BA+1.0 mg/L IAA for subculture, and 1/2 MS+ 0.3 mg/L IBA for rooting culture. The transplanting medium of test-tube plantlet was the mixture of perlite and peat, in which the survival rate was 96%. [Conclusion] The establishment of rapid propagation system of high-quality wolfberry lays good technological base for accelerating breeding progress, breeding and promoting new improved varieties. It is feasible to breed high-quality seedlings of wolfberry through choosing superior strains.
[Objective] To study various factors that affect the rapid propagation of wolfberry (Lycium barbarum L.) in vitro and to establish the rapid propagation system of wolfberry superior strains with the purpose of breeding a large number of high-quality seedlings. [Method] Using Ningqi No. 1 twigs as explants, the sterilization methods, lateral bud induction, subculture, rooting culture mediums of wolfberry were optimized. [Result] Explants were first soaked for 5- The optimized culture mediums were MS + 0.5 mg / L 6-BA + 0.1 mg / L IAA for lateral bud induction, MS + 0.5 mg / L 6-BA + 1.0 mg / L for 8 min in 500 mg / L penicillin injecta then sterilized IAA for subculture, and 1/2 MS + 0.3 mg / L IBA for rooting culture. The transplanting medium of test-tube plantlet was the mixture of perlite and peat, in which the survival rate was 96%. [Conclusion] The establishment of rapid propagation system of high-quality wolfberry lays good techn It is feasible to breed high-quality seedlings of wolfberry through choosing superior strains.