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目的观察分析枸杞多糖对氯化甲基汞所诱导神经干细胞(NSCs)损伤的影响。方法取孕16 dSD大鼠胚胎的海马组织,分离纯化得到海马NSCs。将纯化后的海马NSCs增殖、生长10 d后,按随机分组方法将其分为空白对照组(DMEM/F12培养基);枸杞多糖组(DMEM/F12培养基+枸杞多糖);氯化甲基汞组(DMEM/F12培养基+氯化甲基汞);氯化甲基汞+枸杞多糖组(DMEM/F12培养基+氯化甲基汞+枸杞多糖)。分别测定各组生长环境中的海马NSCs分化、生长的情况,观察分析氯化甲基汞对海马NSCs的损伤作用,以及枸杞多糖干预后对海马NSCs的影响。结果氯化甲基汞组和空白对照组比较,加入氯化甲基汞后的海马NSCs分化后的神经元比例(3.63%±0.62%)和神经元的周长(63.36μm±5.57μm)都较空白对照低;枸杞多糖组和其他各组比较,海马NSCs分化后的神经元比例(7.75%±0.59%)和神经元的周长(253.3μm±11.21μm)都较其他各组高;氯化甲基汞+枸杞多糖组和氯化甲基汞组比较,前者生长环境下的海马NSCs分化后的神经元比例(5.92%±0.98%)和神经元的周长(111.9μm±6.07μm)较氯化甲基汞组高。结论氯化甲基汞对海马NSCs的分化、生长具有损伤作用;枸杞多糖可以减轻氯化甲基汞对海马NSCs的损伤作用,并能促进海马NSCs向神经元的分化及神经元的生长。
Objective To investigate the effect of LBP on the damage of neural stem cells (NSCs) induced by methyl mercuric chloride. Methods The hippocampal tissues of embryonic day 16 SD rats were obtained, and the hippocampal NSCs were isolated and purified. The purified hippocampal NSCs were proliferated for 10 days and divided into blank control group (DMEM / F12 medium), Lycium barbarum polysaccharide group (DMEM / F12 medium + lycium barbarum polysaccharide), methyl chloride Mercury group (DMEM / F12 medium + methylmercury chloride); methylmercury chloride + LBP group (DMEM / F12 medium + methylmercury chloride + LBP). The differentiation and growth of hippocampal NSCs in the growth environment of each group were determined. The damage of hippocampal NSCs induced by methylmercury chloride was observed and analyzed, and the effect of LBP on hippocampal NSCs was also observed. Results Compared with the blank control group, the percentage of NSCs differentiated into neurons (3.63% ± 0.62%) and the circumference of neurons (63.36μm ± 5.57μm) Compared with other groups, the percentage of neurons differentiated into hippocampal NSCs (7.75% ± 0.59%) and neurons (253.3μm ± 11.21μm) were higher in LBP group than in other groups; Compared with methylmercuric chloride group and methylmercury chloride group, the proportion of NSCs differentiated into neurons (5.92% ± 0.98%) and neurons (111.9μm ± 6.07μm) Higher than the methylmercury chloride group. Conclusion Methylmercury chloride has a damaging effect on the differentiation and growth of hippocampal NSCs. Lycium barbarum polysaccharide can reduce the damage of hippocampal NSCs induced by methylmercury chloride and promote the differentiation of neurons and the growth of neurons in hippocampus.