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为探讨温度对茶小绿叶蝉适应能力的影响,室内设置-10、-5、0、5、10、15、20、25、30、35、38、41和44℃共13个温度梯度,测定了不同温度处理1 h后成虫存活率及其保护酶系超氧化物歧化酶(SOD)、铜锌超氧化物歧化酶(Cn Zn-SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、总抗氧化能力(T-AOC)和总蛋白含量的变化。结果表明,在5~35℃之间,成虫存活率均大于90%,其它处理成虫存活率明显降低。SOD、POD、CAT、T-AOC活性和总蛋白含量变化在5~30℃之间差异不显著,温度低于5℃或高于35℃时,保护酶活性亦有降低或增加的趋势,但均以41℃时含量最高,SOD、POD、CAT、T-AOC活性和总蛋白含量分别为2.64、0.68、1.58、0.67 U/mg和178.33μg/m L,但Cn Zn-SOD活性在各处理中均无显著差异,其值在0.96~1.05 U/mg之间。低温处理下的试虫反应更敏感,各指标含量均低于高温处理。表明茶小绿叶蝉成虫存活率、保护酶活性变化、总抗氧化能力和总蛋白含量与温度密切相关,在低温和高温下致死的原因与保护酶系统被破坏有关。
In order to investigate the effect of temperature on the adaptability of green leafhoppers, 13 temperature gradients of -10, -5, 0, 5, 10, 15, 20, 25, 30, 35, 38, 41 and 44 ℃ were set indoors The survival rate and the activities of superoxide dismutase (SOD), copper and zinc superoxide dismutase (Cn Zn-SOD), peroxidase (POD), catalase (CAT), total antioxidant capacity (T-AOC) and total protein content. The results showed that the survival rates of adult adults were more than 90% at 5 ~ 35 ℃, and the survival rates of other adults significantly decreased. The activities of SOD, POD, CAT, T-AOC and the total protein content did not vary significantly from 5 ℃ to 30 ℃. When the temperature was below 5 ℃ or above 35 ℃, the activities of protective enzymes also decreased or increased, The contents of SOD, POD, CAT, T-AOC and total protein were highest at 41 ℃, 2.64,0.68,1.58,0.67 U / mg and 178.33μg / m L, respectively. However, the activity of Cn Zn- No significant difference between the value of 0.96 ~ 1.05 U / mg between. The test insects reaction under the low temperature treatment is more sensitive, the content of each index is lower than the high temperature treatment. The results showed that the survival rate, the change of protective enzyme activity, the total antioxidant capacity and the total protein content were closely related to the temperature, and the causes of death at low temperature and high temperature were related to the destruction of the protective enzyme system.