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【目的】采用高效液相色谱法建立天茄子的华佗豆碱乙的测定方法。【方法】天茄子华佗豆碱乙的检测采用Agilent TCC18柱(4.6 mm×250 mm,5μm),以甲醇—水(含4.5 g/L磷酸氢二钠、1 m L/L三乙胺,2 m L/L磷酸)(体积比为25∶75)为流动相,流速:1.0 m L/min,柱温:30℃,检测波长为243 nm。【结果】华佗豆碱乙在0.11~2.2μg范围内呈良好的线性关系,平均回收率为97.84%,相对标准偏差为0.55%,测定线性关系良好。【结论】该方法可行,结果准确,可用于天茄子的质量控制。
【Objective】 To establish a method for the determination of chrysin-B in Tian-eggplant by high performance liquid chromatography. 【Method】 The test was performed on an Agilent TCC18 column (4.6 mm × 250 mm, 5 μm) with methanol-water (containing 4.5 g / L sodium phosphate dibasic, 1 mL / L triethylamine, 2 m L / L phosphoric acid) (volume ratio of 25:75) as mobile phase at a flow rate of 1.0 mL / min and a column temperature of 30 ℃ with a detection wavelength of 243 nm. 【Result】 The results showed that there was a good linear relationship between Huaibei Beansuan B in the range of 0.11 ~ 2.2μg with an average recovery of 97.84% and a relative standard deviation of 0.55%. The linearity was good. 【Conclusion】 This method is feasible and accurate. It can be used for the quality control of day eggplant.