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目的了解SHP-1基因和SOCS3基因在白血病细胞中的表达及其对预后的影响,为进一步阐明SHP-1和SOCS3基因在白血病发病中的作用机制打下基础。方法用实时荧光定量PCR(FQ-PCR)和RT-PCR方法分别对AL患者和13例正常对照的骨髓进行SHP-1、SOCS3 mRNA水平监测。结果①正常人SHP-1 mRNA均呈阳性表达,其表达水平为3.413±2.018,ALL患者SHP-1均为阴性表达;初治、复发AL组SHP-1表达水平明显低于正常对照组(P<0.01),缓解后SHP-1 mRNA水平上升但仍低于正常对照(P<0.01)。②初治和复发AL患者SOCS3 mRNA表达水平均明显高于缓解组和正常对照组(P<0.05);缓解患者SOCS3 mRNA与正常对照相比无统计学意义(P>0.05)。③SHP-1表达阳性组缓解率(76.4%)明显高于表达阴性组(47.3%)(P<0.01)。结论SHP-1和SOCS3均参与白血病发病,但发病机制不同。SHP-1与白血病的疗效预后有关。
Objective To understand the expression of SHP-1 and SOCS3 in leukemia cells and their effects on prognosis, and lay a foundation for further clarifying the mechanism of SHP-1 and SOCS3 genes in the pathogenesis of leukemia. Methods The levels of SHP-1 and SOCS3 mRNA in bone marrow of AL patients and 13 normal controls were detected by real-time fluorescence quantitative PCR (FQ-PCR) and RT-PCR. Results ① The expression of SHP-1 mRNA in normal controls was 3.413 ± 2.018, while the expression of SHP-1 in ALL patients was negative. The expression of SHP-1 in primary and recurrent AL was significantly lower than that in normal controls <0.01). After remission, the level of SHP-1 mRNA increased but still lower than the normal control (P <0.01). ② The levels of SOCS3 mRNA in newly diagnosed and relapsed AL patients were significantly higher than those in remission and normal controls (P <0.05). There was no significant difference in SOCS3 mRNA between remission patients and normal controls (P> 0.05). ③ The remission rate of SHP-1 positive group (76.4%) was significantly higher than that of negative group (47.3%) (P <0.01). Conclusion Both SHP-1 and SOCS3 are involved in the pathogenesis of leukemia, but their pathogenesis is different. SHP-1 and prognosis of leukemia prognosis.