随着法医DNA分析技术的发展,越来越多低模板量DNA的生物检材被提取,并要求进行STR分型。在此对快速发展的各种低模板量DNA STR分型方法如增加聚合酶链反应(PCR)循环数、纯化PCR扩增产物、激光捕获显微切割和PCR扩增前的全基因组扩增等予以介绍,并就LT-DNA法庭科学应用中存在的相关问题予以论述。 With the development of forensic DNA analysis technology, more and more low-template DNA biological samples are extracted and require STR typing. In this regard, the rapid development of a variety of low-template DNA STR typing methods such as increasing the number of polymerase chain reaction (PCR) cycles, purified PCR amplification products, laser capture microdissection and PCR amplification before genome-wide amplification To introduce and discuss the related problems existing in the application of LT-DNA forensic science.