论文部分内容阅读
首次证实了孕酮和雌二醇这两种类固醇激素在中国对虾体内的存在。在性腺未发育阶段 ,肝胰腺、卵巢和血液中两种激素的含量均很低 ,难以检测到。而在卵黄发生前期 (核仁周边期 ) ,三种组织中孕酮和雌二醇含量迅速上升 ,卵巢的雌二醇含量达到高峰 (45 0 .1± 86 .7)。进入初级卵黄发生阶段 ,三种组织中 ,两种激素均具较高含量 ,卵巢和肝胰腺的孕酮含量 (分别为 1975 .1± 175 .2和 90 2 .6± 130 .5pg/g)以及血淋巴中的雌二醇含量 (45 1.3± 73 .7)达到高峰。到了次级卵黄发生阶段 ,孕酮和雌二醇的含量迅速下降 ,肝胰腺等组织中几乎检测不到。对虾性腺指数 (GSI)的增长既显著且有规律性 ,每一期增长幅度都达到或超过 10 0 %。肝胰腺指数 (HSI)从性腺未发育期 (3 .4± 0 .4)到卵黄发生前期 (4.9± 0 .7)以及从卵黄发生前期到初级卵黄发生期(6 .3± 1.0 )有显著的增长 ,而从初级卵黄发生期到次级卵黄发生期HSI增长不显著 (6 .7± 1.2 )。肝胰腺指数的增长与两种类固醇激素含量的变化具相似的趋势。上述结果显示 ,孕酮和雌二醇可能具有刺激和调控中国对虾性腺发育的作用 ,肝胰腺可能是卵黄蛋白原的合成场所。
For the first time confirmed progesterone and estradiol steroid hormones in the presence of Chinese shrimp. In the undeveloped gonads, both hormones in the hepatopancreas, ovary and blood are low and difficult to detect. In the early stage of the yolk (nucleolus peripheral), the content of progesterone and estradiol in the three tissues increased rapidly, and the level of estradiol in the ovaries peaked (45 ± 1 ± 86.7). Into the primary yolk stage, the three organizations, two hormones were higher levels of progesterone in ovarian and hepatopancreas (1975.1 ± 175.2 and 902.6 ± 130.5pg / g) And the estradiol content in hemolymph (45 1.3 ± 73.7) peaked. To the secondary yolk stage, progesterone and estradiol levels decreased rapidly, almost undetectable in tissues such as hepatopancreas. The growth of shrimp gonadal index (GSI) was significant and regular, with the growth rate of each period reaching or exceeding 10%. Hepatic pancreas index (HSI) was significantly different from that in the gonadal period (3.4 ± 0.4) to the period before the yolk (4.9 ± 0. 7) and from the period before the yolk to the stage of primary yolk (6.3 ± 1.0) While the HSI increase from the primary yolk to the secondary yolk was not significant (6.7 ± 1.2). The increase of hepatopancreas index and the change of two kinds of steroid hormone have a similar tendency. The above results show that progesterone and estradiol may stimulate and regulate gonadal development of P. shrimp. The hepatopancreas may be the synthesis site of vitellogenin.