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目的 探讨脐带间充质干细胞(UC-MSCs)对急性脑缺血/再灌注损伤脑组织内CD31和白细胞介素6 (IL-6)的影响.方法 24只SD 大鼠随机分为对照组、缺血/再灌注组以及缺血/再灌注+干细胞治疗组,每组8只.对照组为仅在颈内动脉下穿线不结扎;缺血/再灌注组为结扎30 min后恢复血流灌注;缺血/再灌注+干细胞治疗组为结扎30 min后恢复血流灌注,并给予干细胞干预治疗,即将2×106脐带干细胞通过尾静脉注射途径输注至大鼠体内.采用免疫组织化学、反转录聚合酶链反应和Western blot技术等检测各组缺血脑组织内CD31和IL-6的变化.结果 三组实验大鼠脑组织中IL-6 mRNA和CD31mRNA及其蛋白表达比较差异均有统计学意义(P<0.05).组间两两比较情况:与对照组相比较,脑缺血/再灌注组大鼠IL-6 mRNA、IL-6蛋白水平升高,缺血/再灌注组+干细胞治疗组降低(P<0.05);与缺血/再灌注组比较,缺血/再灌注组+干细胞治疗组IL-6 mRNA、IL-6蛋白水平降低(P<0.05).与对照组相比较,缺血/再灌注组和缺血/再灌注组+干细胞治疗组的CD31mRNA、CD31蛋白水平均升高,且缺血/再灌注组+干细胞治疗组高于缺血/再灌注组(P<0.05).结论 行UC-MSCs移植术治疗大鼠急性脑缺血/再灌注损伤可有效提高CD31的表达,改善损伤区域血管重建,并通过降低IL-6的表达水平有效抑制炎症进展.“,”Objective To investigate the effects of human umbilical cord mesenchymal stem cells(UC-MSCs)on CD31 and interleukin-6(IL-6)in brains of rats with acute cerebral ischemia /reperfusion injury.Methods A total of 24 SD rats were randomly divided into a control group,a cerebral ischemia/reperfusion group and an ischemia/reperfusion+UC-MSCs group,8 rats each.The control group was only given threading under internal carotid without ligation;in the ischemia/reper-fusion group,blood perfusion was restored after 30 min of ligation vessels blocking blood flow;for the ischemia/reperfu-sion+stem cell therapy group,2 ×106UC-MSCs were infused through the caudal vein,the other operations were same as the ischemia/reperfusion group.The immuno-histochemical method,reverse transcription polymerase chain reaction,Western blot were used to detect the changes of CD 31and IL-6 in the ischemic brain tissues.Results The level of IL-6 mRNA and IL-6 protein of the three groups had significantly differences(P<0.05).Compared to the control group, CD31mRNA,CD31protein were increased significantly in the ischemia/reperfusion group,and decreased in the ischemia/reperfusion+UC-MSCs group(P <0.05);compared to ischemia/reperfusion group,IL-6 mRNA and IL-6 protein were decreased significantly in the ischemia/reperfusion +UC-MSCs group(P<0.05);compared with the control group,the CD31mRNA,CD31protein level in the ischemia/reperfusion group and ischemia/reperfusion +UC-MSCs group were increased significantly,and the ischemia/reperfusion +UC-MSCs group higher than the ischemia/reperfusion group(P<0.05).Conclusion UC-MSCs could effectively increase the expression of CD 31and promote angiogenesis and inhibit the inflammation by the reduction of IL-6 in rats with acute cerebral ischemia/reperfusion injury.