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为探索根区降温条件对葡萄(Vitis vinifera)叶片冻害的影响,以1年生美乐葡萄(V.vinifera cv.‘Merlot’)幼苗为试材,设置根区正常降温和缓冲降温2种降温条件,人工模拟霜冻,分析了叶片冻害指数和叶片的荧光参数。结果表明,根区正常降温会导致根系受冻,同时叶片发生严重的冻害,冻害指数达74.36%;根区缓冲降温使根区温度保持在0°C以上,叶片冻害指数降低53.29%,仅有21.07%的叶片遭受了冻害。根区缓冲降温处理能有效提高叶片霜冻恢复过程中光化学淬灭系数(qP)和天线色素转化效率(F_v~’/F_m~’),加快PSII光合电子传递量子效率(Φ_(PSII))的恢复,提高热耗散能力(NPQ),减轻霜冻后的光抑制(F_v/F_m),有利于叶片霜冻后的恢复。
In order to explore the effect of root zone cooling conditions on frost damage in leaves of Vitis vinifera, two temperature-lowering conditions of normal temperature drop and buffer cooling in root zone were set up with one year old V. vinifera cv.’Merlot ’ , Simulated artificial frost, leaf frost damage index and leaf fluorescence parameters were analyzed. The results showed that the normal root zone cooling would lead to the freezing of the roots and severe frost damage on the leaves with the freezing damage index of 74.36%. The root zone cooling and cooling kept the root zone temperature above 0 ° C and the leaf frost injury index decreased by 53.29% 21.07% of the leaves suffered frost damage. The root zone buffer cooling treatment can effectively increase the photochemical quenching coefficient (qP) and the antennae pigment conversion efficiency (F_v ~ ’/ F_m ~’) and accelerate the recovery of PSII photosynthetic electron transfer quantum efficiency (PSII) , Increasing the heat dissipation capacity (NPQ) and reducing the photoinhibition (F_v / F_m) after frost, which is beneficial to the recovery of the leaves after frost.