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对粗碎矿堆的难选含金硫化矿，用生物氧化方法预处理是一项具有前景的新技术，对不能用加压浸出或焙烧处理的低品位矿石尤其适用。基于矿石的经验生物氧化速率常数、方案的财政贴现率、难选和易选金的品位及金价提出了用于确定矿石中硫化矿最佳生物氧化的代数方程。然后，通过矿石生物氧化速率常数确定矿石生物氧化的最佳分级程度F$，由与F$的对数关系确定生物堆浸预处理经济上最佳的时间F$。为做到这一点，假定矿石连续破碎，生物堆浸预处理，矿石随时间的累积氧化通过矿石试验已知，就能确定速率常数。
Preconditioning gold ore with refractory gold sulphides, which is a refractory ore for coarse crushing mines, is a promising new technology that is particularly suitable for low grade ore that can not be pressure leached or roasted. An algebraic equation for determining the optimal biooxidation of sulphide ores in ore is presented based on the empirical biooxidation rate constant of the ore, the financial discount rate of the scheme, the grade of refractory and readily available gold, and the gold price. Then, the optimal degree of bio-oxidation of ore, F $, is determined by the ore bio-oxidation rate constant and the logarithm of the logarithm of F $ is used to determine the economically optimal time F $ for biopile pretreatment. To do this, the rate constant can be determined on the assumption that the ore is continuously crushed, pre-treated with bio-heap, and the cumulative oxidation of the ore over time is known by ore testing.