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以根瘤超着生大豆突变体0498和完全不结瘤的大豆突变体0701为材料,在人工气候室内模拟条件下,研究大气CO2浓度倍增、UV-B辐射增强以及复合逆境胁迫条件对大豆部分抗性指标的影响。结果表明,大豆突变体0701在上述3种处理下,SOD活性分别下降14.5%、17.4%和25.5%;丙二醛含量在CO2倍增处理下降低29.7%,在UV-B增强处理下则升高18.4%。大豆突变体0498在上述3种处理下,UV-B吸收物质的量分别增加44.4%、39.0%和71.9%;POD酶活分别增加27.3%、47.7%和65.6%;SOD活性在UV-B增强处理下增加27.3%;丙二醛含量在UV-B增强处理下升高85.3%,在复合处理下仅升高62.7%;UV-B增强使其离子渗透率增加12.7%。综合分析可见,突变体0498的抗性优于0701;CO2倍增能提高大豆的抗性,UV-B增强则引起大豆的抗逆反应,复合处理下大豆的响应则较单一处理复杂,无明显规律。
Taking the nodule super shoot mutant 0498 and the complete non-nodulation mutant 0701 as experimental materials, we studied the effect of partial CO2 concentration doubling, UV-B radiation enhancement and composite stress on soybean partial resistance The impact of sexual indicators. The results showed that SOD activity of soybean mutants 0701 decreased by 14.5%, 17.4% and 25.5% respectively under the above three treatments; MDA content decreased by 29.7% under CO2 doubled treatment and increased under enhanced UV-B treatment 18.4%. Soybean mutant 0498 increased the amount of UV-B absorbing substances by 44.4%, 39.0% and 71.9%, POD activity increased by 27.3%, 47.7% and 65.6%, respectively. The activity of SOD increased with the increase of UV-B Treatment increased by 27.3%; MDA content increased by 85.3% UV-B treatment under the composite treatment increased only 62.7%; UV-B enhanced its ion permeability increased by 12.7%. Comprehensive analysis shows that the resistance of mutant 0498 is better than 0701; the doubling of CO2 can increase the resistance of soybean, and the enhancement of UV-B can cause the response of soybean, while the response of soybean under complex treatment is more complicated than single treatment, with no obvious regularity.