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目的 维甲酸类药物调节肿瘤细胞生长、分化和凋亡是其防治肿瘤的基础。本研究观察全反式维甲酸 (ATRA)诱导体外胰腺癌细胞凋亡的可能机制。方法 胰腺癌细胞Patu 8988与ATRA共同孵育。通过DNA断裂分析、TUNEL标记证实凋亡存在 ,并用流式细胞仪检测凋亡细胞比例。用RT PCR和Westernblot方法检测凋亡诱导过程中 p5 3、bcl 2和bax基因的水平及其表达变化。 结果 ATRA处理组细胞凋亡比例明显增加。TUNEL标记和DNA断裂分析发现典型凋亡特征。ATRA处理组bax和phospho p5 3(Ser 4 6 )表达上调 ,但bcl 2表达下调。 结论 ATRA能诱导胰腺癌细胞凋亡 ,其分子机制可能与bcl 2 /bax和 p5 3的表达相关
The purpose of retinoid drugs regulate tumor cell growth, differentiation and apoptosis is the basis of its prevention and treatment of cancer. This study was to investigate the possible mechanism of all-trans retinoic acid (ATRA) -induced pancreatic cancer cell apoptosis in vitro. Methods Pancreatic cancer cell line Patu 8988 was incubated with ATRA. By DNA fragmentation analysis, TUNEL markers confirmed the presence of apoptosis, and the proportion of apoptotic cells was detected by flow cytometry. The levels of p5 3, bcl 2 and bax genes during apoptosis induction were detected by RT PCR and Western blot. Results ATRA treatment group significantly increased the proportion of apoptosis. TUNEL markers and DNA fragmentation analysis revealed typical apoptotic features. ATRA treatment group bax and phospho p5 3 (Ser 4 6) upregulation, but bcl 2 downregulation. Conclusions ATRA can induce the apoptosis of pancreatic cancer cells and its molecular mechanism may be related to the expression of bcl 2 / bax and p5 3